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Objective:To investigate the expression and mechanism of hepatocyte growth factor (HGF) in multiple myeloma (MM) based on the gene information in Oncomine database.Methods:Information about HGF study in Oncomine database was collected, and the changes in HGF expression level in MM were analyzed. Genecards database was used to collect HGF gene-related proteins, and STRING software was used to draw HGF-related protein network map. The physiological process of protein function enrichment was analyzed by using DAVID online tools. The relationship between HGF expression level and survival of MM patients was analyzed by using DRUGSURV database and its online tools to explore its clinical significance.Results:A total of 445 studies on HGF in different tumors were collected in Oncomine database. In 23 studies, the difference in HGF expression level between tumor tissues and normal tissues was statistically significant ( P < 0.05), including 10 items of increased HGF expression in tumor tissues and 13 items of decreased HGF expression in tumor tissues. In 4 datasets of 3 studies on the differential expression of HGF gene in MM and normal tissues in Oncomine database, the expression of HGF in MM tissues was higher than that in normal tissues (all P < 0.05). Twenty-five HGF-related proteins were collected in Genecards database, including SDC1, YWHAG, RAF1, etc. Protein function enrichment analysis showed that these proteins were mainly enriched in the negative regulation of hydrogen peroxide-mediated programmed cell death, the regulation of synaptic plasticity, the negative regulation of death domain receptors on extrinsic apoptotic signaling pathways, etc., and they were related to PI3K-AKT and tumor-related pathways. Survival analysis based on DRUGSURV database showed that there was no significant difference in overall survival rate between MM patients with high and low HGF expression ( P > 0.05). Conclusions:HGF gene may regulate the apoptosis of MM cells through PI3K-AKT pathway and play a role in the occurrence and development of MM. HGF may be a potential marker of MM, but its value in prognostic judgment needs further research.
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Objective:To investigate the expression of the cell cycle mitotic spindle checkpoint serinel/threonine kinase （BUB1） gene in breast cancer and its relationship with the prognosis，and further explore the intervention effect of japonicone A on BUB1 gene in breast cancer cells. Method:Through Oncomine，GEPIA，GEO database，bc-GenExMiner v4.5 and Kaplan-Meier Plotter database， in-depth mining was conducted for BUB1 gene expression-related data，in order to explore the difference in the expression of BUB1 gene in breast cancer tissues and normal breast tissues and its relationship with patient prognosis. Encyclopedia of Cancer Cell Lines （CCLE） was used to analyze the expression of BUB1 in T cells and B cells of breast cancer tissues，STRING database was used to draw BUB1-related protein network diagram and gene ontology（GO） function annotation and analyze relevant pathways in Kyoto Encyclopedia of Genes and Genomes （KEGG）. The tumor immune assessment resource（TIMER） database was used to analyze the expression of BUB1 in immune infiltrates and its impact on the survival and prognosis of patients with gastric cancer. Finally， the effect of japonicone A on the expression level of BUB1 gene in breast cancer cells was further analyzed. Result:①The mRNA level of BUB1 in breast cancer tissues was significantly higher than that of normal tissue samples， but the expression levels of BUB1 in breast cancer patients with different molecular subtypes varied. ② Increased expression of BUB1 could lead to longer distant metastasis-free survival（DMFS），overall survival（OS），and recurrence free survival（RFS） in luminal A subtypes. ③ BUB1 was positively correlated with structural maintenance of chro-mosome 4（SMC4） mRNA expression level， and might be interacted with 10 proteins， such as NUF2， with the strongest interaction relationship. ④ The high expression of BUB1 mRNA in CD8<sup>+ </sup>T cells and neutrophils in breast invasive carcinoma（BRCA） and BRCA-basal had a better two-year survival prognosis than the low expression group；the low expression of BUB1 mRNA in B cells in BRCA-Her2 had a better two-year survival prognosis than the high expression group. ⑤ GEO database analysis data set GSE85871 found that japonicone A could down-regulate the expression of BUB1 gene in breast cancer MCF7 cells. Conclusion:BUB1 gene is highly expressed in breast cancer tissues and related to the prognosis of breast cancer and immune cell infiltration. This may be a new potential therapeutic target for japonicone A to intervene breast cancer cells.
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@#[Abstract] Objective: To explore the expression and biological significance of GABRE gene in colon cancer by mining data in the Oncomine and TCGA databases. Methods: The expression of the GABRE gene in colon cancer tissues and its correlation with the prognosis of patients were analyzed using the Oncomine and TCGA databases. The upstream miRNA targeting GABRE gene was identified using TargetScan, starBase, mirDIP, and miRWalk, and its expression and relationship with prognosis of colon cancer were analyzed. Furthermore, the GABRE co-expression genes were screened using the LinkedOmics database, and the GO enrichment analysis and KEGG pathway analysis were carried out. Results: The results showed that GABRE was highly expressed in colon cancer and indicated a poor prognosis (all P<0.05). The Venn diagram indicated that hsa-miR-370-3p targeted GABRE, and its expression was markedly increased in normal tissues (P<0.01). The expression of GABRE was positively correlated with the expressions of OGT and FAM156A genes, whereas negatively correlated with the expressions of ATP5A1 and MPDU1 genes (all P<0.05). GO biological process function and KEGG pathway enrichment analysis suggested that the GABRE gene may be involved in biological processes including protein dealkylation and regulation of cyclin-dependent protein kinase activity and enriched in taurine metabolism and NF-κB signaling pathway. Conclusions: GABRE gene is highly expressed in patients with colon cancer and indicates a poor prognosis, suggesting that the gene may serve as a potential novel target for the diagnosis and treatment of colon cancer.
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Objective: To explore the expression of Ephrin receptor A7 (EphA7) in the patients with small cell lung cancer (SCLC) and its clinical significance. Methods: The expression of EphA7 mRNA in SCLC cell lines and tissues was analyzed by the Cancer Cell Line Encyclopedia (CCLE) and Oncomine databases, respectively. Seventy-three paraffin-embedded lung cancer specimens and six adjacent normal lung tissue samples from SCLC patients who underwent lobectomy or pneumonectomy resection in Tianjin Medical University Affiliated Cancer Hospital and Institution from January 2011 to December 2014 were collected. The expression of EphA7 protein was assessed by immunohistochemistry. The relationship between the expression of EphA7 protein and other clinicopathological factors was analyzed by x2 test. Kaplan-Meier survival curve and COX proportional hazard model were used to analyze the relationship between these clinicopathological parameters (including EphA7 expression) and the prognosis of SCLC patients. Results: The expression of EphA7 mRNA in SCLC cell lines was the highest among the 1 457 cell lines included in CCLE database. Three datasets of EphA7 mRNA expression in SCLC tissues were obtained from the Oncomine database. Compared with the normal lung tissues and non-small cell lung cancer, the expression level of EphA7 mRNA was relatively higher in SCLC tissues. The positive expression rate of EphA7 protein reached 72.6% (53/73) in the 73 patients with SCLC. The expression of EphA7 protein was significantly associated with lymph node metastasis and TNM stage (both P < 0.05). After adjusting other factors, it was found that the positive expression of EphA7 protein was an independent prognostic factor for the overall survival (OS) of SCLC patients [hazard ratio (HR) = 2.369, 95% confidence interval (CI): 1.075-5.219, P < 0.05], while TNM stage was an independent prognostic factor for both OS (HR = 2.273, 95% CI: 1.252-4.124, P < 0.05) and progression-free survival (PFS) (HR = 1.898, 95% CI: 1.088-3.312, P < 0.05) of SCLC patients, respectively. Conclusion: EphA7 mRNA and protein are highly expressed in SCLC tissues. The expression of EphA7 protein and TNM stage may be independent factors for the prognosis of SCLC patients.
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Objective The objective of this study was to investigate the expression and function of TMEM45A( transmem-brane protein 45A)in clear cell renal cell carcinoma(ccRCC). Methods The data of TMEM45A in Oncomine database were extrac-ted by R language. The relationship between the expression level of TMEM45A and the stage or survival time of ccRCC was analyzed by GEPIA database. qRT-PCR and Western blot were used to detect the expression of TMEM45A in ccRCC tissues and renal cell carcinoma cell lines. After transfection of TMEM45A siRNA,the low expression ofTMEM45A in Caki-1 cells was confirmed by qRT-PCR and Western blot. Cell proliferation after knockdown TMEM45A was analyzed in Caki-1 cells by CCK8. The mechanism of action in the low expression of TMEM45A inhibited cell proliferation was analyzed in Caki-1 cells by qRT-PCR and Western blot. Results A total of 384 TMEM45A-related studies were collected in the Oncomine database,with 35 statistically significant differ-ences,25 of them elevated and 10 decreased. Four studies were associated with ccRCC with a total of 115 samples. The expression of TMEM45A was significantly increased in ccRCC(P<0. 05). It was also found that the high expression of TMEM45A was closely as-sociated with high ccRCC stage and poor prognosis( P<0. 05). When compared with the normal kidney tissues,TMEM45A mRNA was significantly increased in ccRCC tissues(P<0. 05). The expression of TMEM45A at levels of mRNA and protein in Caki-1 and 786-0 cells was higher than that in normal renal tubular epithelial HK-2 cells. After transfection with TMEM45A siRNA for 48h and 72h,the proliferation of Caki-1 cells was significantly decreased(P<0. 001). At the same time,it was found that the expression of PCNA and cyclin D1 at levels of mRNA and protein was significantly decreased ( P <0. 05). Conclusion The expression of TMEM45A is elevated in ccRCC and is associated with ccRCC staging and prognosis. It may be involved in the proliferation of renal carcinoma cells by regulating PCNA and Cyclin D1.
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<p><b>Objective</b>To explore the expression of I-5α-reductase (SRD5A1)and its prognostic role in prostate cancer .</p><p><b>METHODS</b>Data about SRD5A1 were retrieved from the ONCOMINE database and the role of SRD5A1 in prostate cancer was analyzed.</p><p><b>RESULTS</b>Totally, 992 studies of different types relevant to the expression of SRD5A1 were identified in the ONCOMINE database. The SRD5A1 expression was statistically significant in 239 of the studies, overexpressed in 157 (11 in prostate cancer) and underexpressed in the other 82 (3 in prostate cancer). Eighteen of the studies, with 1 068 samples, addressed the expression of SRD5A1 in prostate cancer and normal tissues, which was significantly higher in the former than in the latter tissue (P<0.05). In 3 of the studies, the SRD5A1 expression was high in primary prostate cancer and increased with its metastasis (P<0.0 5). Two of the studies with prognostic data showed a higher rate of postoperative biochemical recurrence and a higher total mortality rate in the patients with a high than in those with a low expression of SRD5A1 (P<0.05).</p><p><b>CONCLUSIONS</b>SRD5A1 is highly expressed in prostate cancer, especially in metastatic and castration-resistant prostate cancer and its expression is associated with the prognosis of prostate cancer, which may be an important target of medication for prostate cancer.</p>
Subject(s)
Humans , Male , 3-Oxo-5-alpha-Steroid 4-Dehydrogenase , Metabolism , Data Mining , Neoplasm Recurrence, Local , Prognosis , Prostatic Neoplasms , Mortality , Pathology , General Surgery , Prostatic Neoplasms, Castration-ResistantABSTRACT
The cell cycle is a conserved process from yeast to mammals and focuses on mechanisms that regulate the timing and frequency of DNA replication and cell division. The temporal and spatial expression of the genes is tightly regulated to ensure accurate replication and transmission of DNA to daughter cells during the cycle. Although the genes involved in interphase are well studied, most of the genes which are involved in mitotic events still remain unidentified. Since, the discovery of mitosis related genes is still incomplete, we performed a co-expression and gene ontology analysis for revealing novel mitosis regulated genes. In this study, we showed that C12orf48 is co-expressed with well-known mitotic genes. Moreover, it is also co-expressed with the genes that have roles in interphase such as DNA replication. Furthermore, our results showed that C12orf48 is also differentially expressed in various cancers. Therefore, the results presented in this study suggest that C12orf48 may be an important molecule for both interphase and mitosis. Since, the molecules involved in these mechanisms are crucial for proliferation as well as in carcinogenesis, C12orf48 should be considered as a novel cell cycle and carcinogenesis related gene.